Laboratory Technician - Axle

Laboratory Technician for NIH/NINDS/Viral Immunology and Intravital Imaging Section (VIIIS) to perform a variety of laboratory techniques and procedures, especially managing mice colonies.

• Work products and documents related to performing laboratory techniques and procedures, including handling mice and infectious agents, performing immune cell isolations, immunohistochemistry, tissue sectioning, fluorescence and bright field microscopy. - Daily
• Clean Equipment - Weekly
• Work products and documents related to performing digital flow cytometry, ELISAs, DNA/RNA isolations, PCR, RT-PCR, quantitative PCR and Western blots - Weekly
• Work products and documents related to working with staff on the maintenance of transgenic mouse colony, cell lines, logs and database. - Daily
• Work products and documents related to inventory supplies and equipment; prepare purchase requests. - Weekly

• Perform a variety of laboratory techniques and procedures, including handling infectious agents, performing immune cell isolations, immunohistochemistry, tissue sectioning, fluorescence and brightfield microscopy 1
• Work with staff on the maintenance and screening of transgenic mouse colony 2
• Work with staff on the maintenance of laboratory cell lines 3
• Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
• Perform digital flow cytometry, ELISAs, DNARNA isolations, PCR, RT-PCR, quantitative PCR and Western blots
• Work with staff on the maintenance of laboratory logs and databases as well as prepare inventory of laboratory supplies and equipment, make recommendations, and prepare purchase requests
• Operates laboratory machines and instruments; and prepares specimens and equipment for use.
• Prepare materials and equipment for experiments.
• Process and organize multiple samples simultaneously.
• Work with a variety of routine and specialized laboratory instruments and equipment in the laboratory.
• Ensure that all specimens and equipment necessary for experiments are available and in good order.
• Prepare specimens and equipment for use in the laboratory.
• Performs sample management and storage.
• Store new samples, update database, retrieve samples with proper authorization.
• Perform processing, description and analysis of samples.
• Work with staff to implement storage and distribution of biological samples for future scientific research.
• Analyzes, documents and reports on all procedures performed.
• Convey and discuss research results with team lab members.
• Collect and enter research data into laboratory notebooks and databases.
• Collect data and prepare for analysis and interpretation.
• Maintains inventory of laboratory supplies.
• Ensure that supplies and consumables are ready and on hand as necessary for maximum efficiency in the laboratory.
• Cleans and sterilizes instruments, glassware and equipment.
• Ensure that adequate clean inventory is available for immediate usage.
• Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
• Perform fundamental procedures required for research projects.
• Perform a variety of laboratory techniques and procedures.
• Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/immunohistochemistry.
• Perform experiments on samples according to protocols.
• Perform routine experiments and tests; follow established procedures and protocols.
• Maintains inventory of laboratory supplies and initiates purchase orders.
• Work with staff to maintain stocked supplies and records of working reagents, including fixatives, buffers, solvents and staining.
• Ensure that reagents are made correctly and that expired reagents or defective products are replaced to ensure validity of results.
• Order and maintain laboratory supplies, reagents and equipment.
• Performs routine equipment maintenance.
• Assist in troubleshooting and calibrating various laboratory equipment and instruments.
• Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
• Perform a variety of laboratory techniques and procedures.
• Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/immunohistochemistry.
• Perform routine experiments and tests; follow established procedures and protocols.
• Analyzes, documents and reports on all procedures performed and produces detailed reports for use by senior staff.
• Collect, analyze and interpret data; prepare summaries and reports based on results and findings.
• Record details of experimental procedures, results and protocols.
• Performs advanced equipment maintenance.
• Assist in troubleshooting and calibrating various laboratory equipment and instruments.
• Trains staff in performing routine laboratory tests and procedures.
• Provide technical help and expertise to lab members.
• Work with staff to monitor and ensure that appropriate laboratory safety courses and certifications for staff are up to date.

• Bachelor's degree in Biology, Life Sciences or a related discipline
• Our Field of Study is Neuroimmunology

• Neuroscience

• NIH POTS
• Microsoft Outlook, Word, Excel, and Powerpoint

• Perform a variety of laboratory techniques and procedures, including handling infectious agents, performing immune cell isolations, immunohistochemistry, tissue sectioning, fluorescence and brightfield microscopy
• Maintain laboratory cell lines
• Maintain and screen transgenic mouse colony