Associate Scientist, Molecular Biology and Assay Development - Eurofins DiscoverX Products, LLC

Company Description

Eurofins Scientific is an international life sciences company, providing a unique range of analytical testing services to clients across multiple industries, to make life and our environment safer, healthier and more sustainable. From the food you eat, to the water you drink, to the medicines you rely on, Eurofins works with the biggest companies in the world to ensure the products they supply are safe, their ingredients are authentic and labelling is accurate.

Eurofins is the global leader in food, environment, pharmaceutical and cosmetic product testing and in agroscience Contract Research Organisation services. Eurofins is one of the market leaders in certain testing and laboratory services for genomics, discovery pharmacology, forensics, advanced material sciences and in the support of clinical studies, as well as having an emerging global presence in Contract Development and Manufacturing Organisations. The Group also has a rapidly developing presence in highly specialised and molecular clinical diagnostic testing and in-vitro diagnostic products.

In over just 30 years, Eurofins has grown from one laboratory in Nantes, France to 55,000 staff across a decentralised and entrepreneurial network of 900 laboratories in over 50 countries. Eurofins offers a portfolio of over 200,000 analytical methods to evaluate the safety, identity, composition, authenticity, origin, traceability and purity of biological substances and products.

In 2020, Eurofins generated total revenues of EUR € 5.4 billion, and has been among the best performing stocks in Europe over the past 20 years.

Job Description

Become your most extraordinary self! Our team is intensely proud to be a major provider of services and products to the drug discovery research industry. Join Eurofins Panlabs, part of the Eurofins Discovery ™ group of companies serving serving global clients in the Pharma and Biotech industries. Work alongside industry experts and collaborate with team members to directly and positively impact human health worldwide!

We have a philosophy to support work life balance, career progression opportunities and offer many benefits such as formal mentoring programs, tuition reimbursement, extensive career training programs, competitive health benefits, paid time off and 10 annual paid holidays.

The Associate Scientist, Molecular Biology and Assay Development, is an integral member of the Eurofins DiscoverX R&D Department. They will support all departmental molecular biology and cell line development activities by executing molecular cloning of expression vectors, characterization / validation of cell lines and gene delivery to cells using viral and other methods. They will also participate in generating cell lines and/or running cell-based assays using various technologies, including Eurofins DiscoverX's Enzyme Fragment Complementation (EFC).

Essential Duties and Responsibilities:

• Actively maintain the Eurofins DiscoverX plasmid collection including fulfilling requests for plasmids and generating additional quantities of plasmids by performing DNA preps (in-house or via out-sourcing).
• Design and generate mammalian expression constructs: identify the correct gene sequence (according to the workplan); optimize the DNA sequence (codon usage) and confirm in-frame fusion with tags (if applicable).
• Use bioinformatics tools (including SnapGene, NCBI databases, etc) for executing and confirming cloning projects.
• Routinely submit samples to, and evaluate data returned by, our external DNA sequencing partner
• Perform PCR on genomic DNA from cell lines to confirm stable integration of genes and quantitative PCR to confirm gene expression.
• Perform RNA extraction and quantify gene expression by qRT-PCR.
• Generate Biosafety Documentation.
• Perform routine cell culture, as well as stable pool and clone development of engineered cells.
• Perform characterization of engineered cell lines using molecular, biochemical, and cell biology techniques, including flow cytometry, qPCR, Western blotting, immunofluorescence or other functional readouts.
• Perform cell-based assays using established Eurofins DiscoverX assays to test activity of client molecules and report results to manager in appropriate format(s).
• Accurately prepares and presents experimental data at regular group meetings.
• Works diligently to meet product development goals set by management team.
• Provides support functions to the R&D team such as ordering and maintenance of reagents/supplies/equipment, maintaining accurate notes and records, and effective communication.
• Performs all duties in compliance with all company procedures and regulations.
• Ability to