Laboratory Neuroscientist Technician - Axle

• Work products related to screening for autoantibodies in human biofluids (blood fractions and cerebrospinal fluid) using a variety of methods including rodent (and non-human primate) brain tissue immunostaining, human protein array, iPSC-derived neuronal culture and immunostaining, and (less commonly) immunoprecipitation mass spectrometry (IP-MS). - Daily
• Work products related to Assist in troubleshooting and calibrating various laboratory equipment and instruments. - Daily
• Work products related to Collect, analyze and interpret data; prepare summaries and reports based on results and finding. - Daily

• Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research. 
• Draft and maintain standard operating procedures for antibody screens and testing.
• Operates laboratory machines and instruments; and prepares specimens and equipment for use. 
• Maintain electronic biospecimen handling and testing logs.
• Performs sample management and storage. 
• Schedule regular maintenance of equipment logs and maintain performance logs.
• Analyzes, documents and reports on all procedures performed. 
• Ensure that reagents are made correctly and that expired reagents or defective products are replaced to ensure validity of results.
• Maintains inventory of laboratory supplies. 
• Sacrifice, perfuse, and cryopreserve mouse brain tissue.
• Cleans and sterilizes instruments, glassware and equipment. 
• Immunostaining rodent and/or NHP brain tissue with human CSF, serum/plasma, and commercial antibodies.
• Plan, execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research. 
• Preparation of presentation slides and figures for outside investigators.
• Occasionally attend lab meetings of outside investigators.
• Maintains inventory of laboratory supplies and initiates purchase orders. 
• Assist in troubleshooting and calibrating various laboratory equipment and instruments. 
• Collect, analyze and interpret data; prepare summaries and reports based on results and findings.
• Performs routine equipment maintenance. 
• Primary cell isolation and culture (neurons, astrocytes, oligodendrocytes, and mixed cell culture). 
• iPSC-derived neuronal cell differentiation and culture.
• Plan, execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.  
• Perform experiments on samples according to protocols. 
• Logging of assay and experimental results in electronic laboratory notebook (Lab Archives).
• Analyzes, documents and reports on all procedures performed and produces detailed reports for use by senior staff. 
• Human protein array immunostaining (HuProt) and data processing. 
• Performs advanced equipment maintenance. 
• Screening for autoantibodies in human biofluids (blood fractions and cerebrospinal fluid) using a variety of methods including rodent (and non-human primate) brain tissue immunostaining, human protein array, iPSC-derived neuronal culture and immunostaining, and (less commonly) immunoprecipitation mass spectrometry (IP-MS). 
• Trains staff in performing routine laboratory tests and procedures. 
• Expected to engage with outside investigators for the purpose of interpreting and contextualizing preliminary results.
• Provides insights and expertise and recommends new strategies and approaches. 

Certifications & Licenses:

• Clinical Laboratory Improvement Amendments (CLIA) 

Field of Study:

• Microbiology 
• Molecular Biology 

Software:

• PowerPoint 
• MS Project 
• MS Access 

Skills:

• Experience in CLIA-Certified Lab 
• Experience in Rodent Handling and Husbandry 
• Expertise in Cell Line and Primary Cell Culture 
• Expertise in Small Rodent Sacrifice, Perfusion, Cryopreservation, and Paraffin Embedding 
• Expertise in Immunostaining: Immunofluorescence, DAB, and Colorimetric 
• Animal Handling: Non-Human Primates