Research Assistant- RI Microbial Pathogen King - Nationwide Children's Hospital

We are seeking a motivated, organized, hard-working recent graduate with some research lab experience. While the majority of the successful applicant’s time will be spent at the bench, we believe in teaching all aspects of science including science writing, practical skills, data analysis, ability to think critically about your work, ability to critically evaluate literature and presentation skills. The applicant will be responsible for every aspect of their own project under direct supervision of a postdoctoral scientist and Dr. King. Our research is focused on bacteria in the genus Streptococcus. These species cause a wide range of human diseases including pneumonia, bacteremia, otitis media and endocarditis and are major causes of morbidity and mortality world-wide. Development of more effective vaccines and treatments will require a greater understanding of the pathogenic mechanisms utilized by these organisms. Glycans decorate all host surfaces and many host molecules, and as such can play a critical role in the disease process. Dr. King’s laboratory studies how alpha-hemolytic streptococci modify and interact with host glycans and how these interactions contribute to colonization and pathogenesis. Current studies focus on how endocarditis pathogen S. oralis interacts with glycans in the oral cavity and in the heart to cause disease.

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PRINCIPAL DUTIES AND RESPONSIBILITIES

• Performs research experiments independently or semi-independently in accordance with protocols approved by the principal investigator. Such experiments may involve the use of laboratory animals or human subjects any may require evening and/or weekend duties. 
• Prepares and tests substrate, conducts assays, and prepares tissue cultures for use in research studies, as applicable. Collects blood, urine, and other samples as needed. Such activities may be performed independently and may require evening and weekend hours. 
• Ensures accuracy of laboratory analytical procedures as required by specific research projects. 
• Collects and controls experimental data and patient information; and maintains accurate daily logs of experiments including methods, results, and conclusions. 
• Interprets the results of data analyses with input from the principal investigator. 
• Assists the principal investigator in the preparation of scientific publications and grant proposals. 
• Make recommendations about refinement and/or replacement of laboratory analytical procedures. Implements such refinements or new procedures with input from the principal investigator.
• Orders supplies needed to conduct research; maintains inventory of equipment and supplies; and assures that research equipment is in good working order. 
• Collaborates in the preparation of research budgets, and monitors expenditure of funds to assure that studies are conducted within budgetary constraints. 
• Instructs other laboratory workers and students in standard methods, practices, procedures, and equipment operations. 
• Maintains a clean working area and performs general laboratory maintenance. Adheres to proper safety techniques and procedures. 
• Performs miscellaneous related duties.

KNOWLEDGE , SKILLS AND ABILITIES REQUIRED

• Bachelor of Science degree with two to three years of laboratory research experience OR certification and experience may be considered in lieu of formal education. Laboratory experience preferred Coursework in anatomy, physiology, statistics, relevant research methods and radiation desirable.

MINIMUM PHYSICAL REQUIREMENTS

Percent of time spent on: 50% Preparing test substrates, conducting assays, preparing cultures 25% Interpreting results of data analyses with PI 11% Miscellaneous paperwork associated with research 10% Ordering supplies 02% Preparation of budget 02% Instructing other lab personnel Minimum physical requirements: 100% Kneeling / reaching 100% Finger dexterity 100% Talking / hearing / seeing 33% Lifting / carrying weight 0-20 pounds for 1-5 minutes The above list of duties is intended to describe the general nature and level of work performed by individuals assigned to this classification. It is not to be construed as an exhaustive list of duties performed by the individual so classified, nor is it intended to limit or modify the right of any supervisor to assign, direct, and control the work of employees under his/her supervision. EOE M/F/Disability/Vet