Research Scientist/Engineer 2, Long-Read Sequencing. - University of Washington

Req #: 238930 Department: GENOME SCIENCES Appointing Department Web Address: https://www.gs.washington.edu/index.htm Job Location Detail: This is an in-person position. Posting Date: 10/03/2024 Closing Info: Open Until Filled Salary: $4,649 - $7,500 per month Shift: First Shift Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230701\_a11y.pdf ) As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. The University of Washington (UW) is proud to be one of the nation's premier educational and research institutions. Our people are the most important asset in our pursuit of achieving excellence in education, research, and community service. Our staff not only enjoys outstanding benefits and professional growth opportunities, but also an environment noted for diversity, community involvement, intellectual excitement, artistic pursuits, and natural beauty. The Department of Genome Sciences (GS) is part of the University of Washington School of Medicine (UW SOM) and is comprised of 31 labs addressing leading edge questions in biology and medicine by developing and applying genetic, genomic and computational approaches taking advantage of genomic information now available for a host of species. GS is making major contributions in the application of genome science to understand the fundamentals of biology and to improve human health and wellbeing. Genome Sciences is a collaborative department supported by very different academic disciplines. We work with a multitude of Program Investigators (PIs) in departments both on the UW campus as well as worldwide. The department has gone through significant expansion since its beginning in 2001 with annual grant awards of $63M in FY23. The department has 62 training, teaching, and research faculty. The Department of Genome Sciences is committed to creating an environment that is welcoming and inclusive. Our goal is to foster a place of learning and working where all members can thrive and where diversity is recognized and celebrated. At Genome Sciences, we aim to create supportive spaces for those who are marginalized in higher education or society. To achieve this, we recognize that it is our ongoing responsibility to understand, acknowledge, and challenge systems of privilege and disadvantage in higher education such as those based on race, color, creed, caste, religion, national origin, citizenship, sex, age, marital status, sexual orientation, gender identity or expression, disability, veteran status, or socioeconomic status. **POSITION PURPOSE** This is an exciting opportunity for a **Research Scientist/Engineer 2 (RSE2)** to work with new long-read DNA and RNA sequencing technology. Projects will include using long-read sequencing technology to investigate the role of genomic structural variation in human disease, performing DNA and RNA sequencing of individuals with unsolved genetic disorders, and collaborating with a diverse group of investigators to design and implement long-read sequencing projects. The position will be actively involved in development of cutting-edge techniques in next-generation and long-read DNA and RNA sequencing with a focus on RNA based projects, for projects both within the laboratory and with outside collaborators. **POSITION SUMMARY** The RSE2 will actively contribute to ongoing laboratory research projects and work with local and international collaborators to design and implement long-read sequencing projects on the Pacific Biosciences (PacBio) Revio platform. Work collaboratively with other technicians; assist graduate students and postdoctoral fellows with ongoing research projects. Independently perform scientific investigative procedures under limited supervision. Complete assigned tasks on time to achieve tight production deadlines. The successful candidate will have strong multitasking skills and an interest in scientific inquiry on the technical scale in addition to broader thinking. **DUTIES AND RESPONSIBILITIES** + Perform sequencing projects on PacBio Revio sequencing platform. Includes library preparation, sequencer operation, data quality monitoring, and basic data analysis. Full hands-on training provided, if necessary. 40% + RNA sample QC, processing, and library preparation from RNA. 25% + Mammalian cell tissue culture in a BSL-2 environment. 10% + DNA and RNA isolation from various sources including cell culture, tissue, and blood. 5% + Assist researchers with experimental design, project updates, data delivery, and analysis questions via email and telephone correspondence. 5% + Sample tracking and database maintenance. 5% + Perform routine analysis of data using the command line: assist with troubleshooting and daily quality control of data and assist researchers with data analysis, organization and interpretation, and experimental design. 5% + Perform related duties as required. 5% **MINIMUM REQUIREMENTS** + Bachelor's degree in Microbiology, Genetics or closely related Life Sciences field + 2 years' experience and knowledge of the fundamental concepts, practices and procedures required for working in a genomics laboratory. **Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.** **ADDITIONAL REQUIREMENTS** + Exposure to next-generation sequencing and genomics laboratory techniques. + Competence with RNA extraction and handling protocols + Competence in basic computing skills using Microsoft Word, Excel, and PowerPoint, or Google Docs and Sheets. + Ability to work independently and as part of a multidisciplinary team. + Ability to learn new techniques. + Attention to detail and strong organizational abilities. + Experience with DNA & RNA isolation from various sources. + Or Equivalent combination of education/experience . **DESIRED QUALIFICATIONS** + Master's degree in Microbiology, Genetics or related Life Sciences field. + Familiarity with human genomics terms and concepts. + Some knowledge of sequence analysis, programs, and programming for bioinformatics. + Experience with UNIX/*NIX command line, BASH shell scripting, and Python. + Demonstrated commitment to valuing diversity and contributing to an inclusive working and learning environment. **Application Process:** The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select "Apply to this position". Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your "My Jobs" page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed. University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.